intravascular hemolysis associated with candidatus mycoplasma hematoparvum in a non-splenectomized dog in the south region of iran

a 2-year-old male pekingese dog was referred to shiraz university’s veterinary teaching hospital for anorexia and depression. the case had no history of surgery. physical examination revealed no abnormalities except mild depression and fever. small, coccoid, epicellular bacteria were detected on erythrocytes by microscopic examination of the giemsa-stained blood smears. abnormalities noted in the complete blood count included regenerative anemia characterized by a marked reticulocytosis. examination of the plasma showed visual evidence of slight intra vascular hemolysis. in addition, howell-jolly bodies, nucleated rbcs, increased immature neutrophils and thrombocytosis were found in this case. the urine was strongly positive for bilirubin, and the urine sediment had abundant bilirubin crystals. for polymerase chain reaction (pcr) purpose, total dna was extracted from blood sample collected from dog. pcr was positive and phylogenetic analysis of concatenated data showed our isolate clustered within candidatus mycoplasma hematoparvum group. treatment was performed by oral ciprofloxacin and prednisolone. the clinical signs improved after three days. two month follow-up showed no recurrence. in conclusion, hemoplasmosis should be considered as a differential diagnosis in dogs with hemolytic process and pyrexia. the pcr evaluation for hemoplasma dna should be included in the investigation of such cases to enable the rapid detection of this infection, which may be more common than previously estimated. besides, ciprofloxacin might have an effect on treatment of hemoplasma in dogs, however, conducting further case studies are necessary to recommend successful treatment.